Journal: Biological Research
Article Title: Establishment of primary prostate epithelial and tumorigenic cell lines using a non-viral immortalization approach
doi: 10.1186/s40659-024-00507-z
Figure Lengend Snippet: In vivo tumor formation ability of immortalized cell lines. A Tumors derived via injection of prostate cancer cell line 22Rv1 and newly derived cell line MS-pPC-193ST in SCID mice. 22Rv1 tumors and MS-pPC-193ST tumors were resected 2 weeks and 14 weeks after injection, respectively (Bars represent 1 cm). B Hematoxylin and eosin staining (upper panel) revealed prostatic adenocarcinoma in 22Rv1 tumors, whereas MS-pPC-193ST tumors displayed squamous differentiation. Middle panel: 22Rv1 showed strong nuclear AR expression, whereas no AR expression was observed in MS-pPC-193ST tumors. Lower panel: MS-pPC-193ST tumors displayed nuclear expression of SV40LT, which was absent in 22Rv1 tumors (magnification: 10X; bars represent 200 µm)
Article Snippet: The human metastases derived prostate cancer cell lines 22Rv1, LNCaP and PC-3 were purchased from the Leibniz-Institute DSMZ GmbH (Braunschweig, Germany).
Techniques: In Vivo, Derivative Assay, Injection, Staining, Expressing